MaghrebDataLibMedV2, Main, Exploration, bibRecord, 000617

Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.

Identifieur interne : 000617 ( Main/Exploration ); précédent : 000616; suivant : 000618

Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.

Auteurs : Nadia Taïbi [Algérie] ; Amina Taïbi [Algérie] ; Rachid Ameraoui [Algérie] ; Mohamed Abou-Mustapha [Algérie] ; Mohamed Hadjadj [Algérie] ; Zahra-Mouna Boutaiba [Algérie] ; Amel Kaced [Algérie] ; Souhila Djema [Algérie] ; Qosay-Ali Al-Balas [Jordanie] ; Ghazi-Ahmad Al Jabal [Jordanie] ; Miriem Aissi [Algérie] ; Khaled Harhoura [Algérie] ; Safia Zenia [Algérie] ; Farida Khammar [Algérie]

Source :

Biochimie [ 1638-6183 ] ; 2020.

RBID : pubmed:31707099

Descripteurs français

KwdFr :
- Animaux (MeSH), Bovins (MeSH), Chromatographie en phase liquide à haute performance (méthodes), Chromatographie gazeuse-spectrométrie de masse (méthodes), Fasciola hepatica (isolement et purification), Fasciolase (diagnostic), Fasciolase (médecine vétérinaire), Femelle (MeSH), Marqueurs biologiques (sang), Mâle (MeSH), Méthylglyoxal (sang).
MESH :
- diagnostic : Fasciolase.
- isolement et purification : Fasciola hepatica.
- médecine vétérinaire : Fasciolase.
- méthodes : Chromatographie en phase liquide à haute performance, Chromatographie gazeuse-spectrométrie de masse.
- sang : Marqueurs biologiques, Méthylglyoxal.
- Animaux, Bovins, Femelle, Mâle.

English descriptors

KwdEn :
- Animals (MeSH), Biomarkers (blood), Cattle (MeSH), Chromatography, High Pressure Liquid (methods), Fasciola hepatica (isolation & purification), Fascioliasis (diagnosis), Fascioliasis (veterinary), Female (MeSH), Gas Chromatography-Mass Spectrometry (methods), Male (MeSH), Pyruvaldehyde (blood).
MESH :
- chemical , blood : Biomarkers, Pyruvaldehyde.
- diagnosis : Fascioliasis.
- isolation & purification : Fasciola hepatica.
- methods : Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry.
- veterinary : Fascioliasis.
- Animals, Cattle, Female, Male.

Abstract

Two analytical methods; high performance liquid chromatography and gas chromatography were used to determine the content of 2-methylquinoxaline, a methylglyoxal-derived agent in sera from cattle with fascioliasis. Methylglyoxal is a highly mutagenic and cytotoxic reactive dicarbonyl compound formed by non-enzymatic fragmentation of triose phosphate GAP and DHAP during glycolysis which regularly contributes to repositioning the energetic balance between physiological and pathological situations. The aim of this study was to propose the MGO as a new biomarker in the bovine fasciolosis. Strongly infected animals showed a correlation between the relatively high levels of Fasciola hepatica anti-f2 antibody and methylglyoxal compared to unharmed animals. Also, an acute hyperglycemia was recorded and closely related to hepatic parenchyma hyperplasia, inflammation, bile ducts obstruction and scléro-fibrous foci formation.Unlike HPLC, which has shown analytical flaws and irregularities, GC-MS remains an excellent diagnostic tool for detecting and quantifying methylglyoxal in biological fluids. The developed method has been validated under FDA guidelines. A full scan-range was set from m/z 39 to 144/999 and the molecular weight of the 2-methylquinoxaline was identified according to NIST Database and ES. Methylglyoxal was the only analyte successfully quantified in a relatively short run time. It was linear over a concentration range of 0.057-5.7  μg.ml-1with mean recoveries and RSD of 118% and 3.63% respectively. The intra and inter-day assays were satisfying and not exceed 3.00%. Results reflect the degree of precision of our method and indicate that MGO was an important contributor to understand the hepatic failure independently of other serum markers.

DOI: 10.1016/j.biochi.2019.11.002
PubMed: 31707099

Affiliations:

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Le document en format XML

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<author><name sortKey="Abou Mustapha, Mohamed" sort="Abou Mustapha, Mohamed" uniqKey="Abou Mustapha M" first="Mohamed" last="Abou-Mustapha">Mohamed Abou-Mustapha</name>
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<author><name sortKey="Hadjadj, Mohamed" sort="Hadjadj, Mohamed" uniqKey="Hadjadj M" first="Mohamed" last="Hadjadj">Mohamed Hadjadj</name>
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<author><name sortKey="Boutaiba, Zahra Mouna" sort="Boutaiba, Zahra Mouna" uniqKey="Boutaiba Z" first="Zahra-Mouna" last="Boutaiba">Zahra-Mouna Boutaiba</name>
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<author><name sortKey="Kaced, Amel" sort="Kaced, Amel" uniqKey="Kaced A" first="Amel" last="Kaced">Amel Kaced</name>
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<author><name sortKey="Djema, Souhila" sort="Djema, Souhila" uniqKey="Djema S" first="Souhila" last="Djema">Souhila Djema</name>
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<author><name sortKey="Al Balas, Qosay Ali" sort="Al Balas, Qosay Ali" uniqKey="Al Balas Q" first="Qosay-Ali" last="Al-Balas">Qosay-Ali Al-Balas</name>
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<country xml:lang="fr">Jordanie</country>
<wicri:regionArea>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid</wicri:regionArea>
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<author><name sortKey="Al Jabal, Ghazi Ahmad" sort="Al Jabal, Ghazi Ahmad" uniqKey="Al Jabal G" first="Ghazi-Ahmad" last="Al Jabal">Ghazi-Ahmad Al Jabal</name>
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<author><name sortKey="Aissi, Miriem" sort="Aissi, Miriem" uniqKey="Aissi M" first="Miriem" last="Aissi">Miriem Aissi</name>
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<author><name sortKey="Harhoura, Khaled" sort="Harhoura, Khaled" uniqKey="Harhoura K" first="Khaled" last="Harhoura">Khaled Harhoura</name>
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<author><name sortKey="Zenia, Safia" sort="Zenia, Safia" uniqKey="Zenia S" first="Safia" last="Zenia">Safia Zenia</name>
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<country xml:lang="fr">Algérie</country>
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<author><name sortKey="Khammar, Farida" sort="Khammar, Farida" uniqKey="Khammar F" first="Farida" last="Khammar">Farida Khammar</name>
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<wicri:regionArea>Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111</wicri:regionArea>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.</title>
<author><name sortKey="Taibi, Nadia" sort="Taibi, Nadia" uniqKey="Taibi N" first="Nadia" last="Taïbi">Nadia Taïbi</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria; Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: Nadbio71.doc@gmail.com.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria; Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111</wicri:regionArea>
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<author><name sortKey="Taibi, Amina" sort="Taibi, Amina" uniqKey="Taibi A" first="Amina" last="Taïbi">Amina Taïbi</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aminataibi.ensv@yahoo.com.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
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<author><name sortKey="Ameraoui, Rachid" sort="Ameraoui, Rachid" uniqKey="Ameraoui R" first="Rachid" last="Ameraoui">Rachid Ameraoui</name>
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<country xml:lang="fr">Algérie</country>
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<wicri:noRegion>Tipaza</wicri:noRegion>
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</author>
<author><name sortKey="Abou Mustapha, Mohamed" sort="Abou Mustapha, Mohamed" uniqKey="Abou Mustapha M" first="Mohamed" last="Abou-Mustapha">Mohamed Abou-Mustapha</name>
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<country xml:lang="fr">Algérie</country>
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<wicri:noRegion>Tipaza</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hadjadj, Mohamed" sort="Hadjadj, Mohamed" uniqKey="Hadjadj M" first="Mohamed" last="Hadjadj">Mohamed Hadjadj</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mohhadj40@yahoo.fr.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza</wicri:regionArea>
<wicri:noRegion>Tipaza</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boutaiba, Zahra Mouna" sort="Boutaiba, Zahra Mouna" uniqKey="Boutaiba Z" first="Zahra-Mouna" last="Boutaiba">Zahra-Mouna Boutaiba</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mounaboutaiba@yahoo.fr.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza</wicri:regionArea>
<wicri:noRegion>Tipaza</wicri:noRegion>
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<author><name sortKey="Kaced, Amel" sort="Kaced, Amel" uniqKey="Kaced A" first="Amel" last="Kaced">Amel Kaced</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: k.amel07@yahoo.fr.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza</wicri:regionArea>
<wicri:noRegion>Tipaza</wicri:noRegion>
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</author>
<author><name sortKey="Djema, Souhila" sort="Djema, Souhila" uniqKey="Djema S" first="Souhila" last="Djema">Souhila Djema</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: gensou85@hotmail.com.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza</wicri:regionArea>
<wicri:noRegion>Tipaza</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Al Balas, Qosay Ali" sort="Al Balas, Qosay Ali" uniqKey="Al Balas Q" first="Qosay-Ali" last="Al-Balas">Qosay-Ali Al-Balas</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: qabalas@just.edu.jo.</nlm:affiliation>
<country xml:lang="fr">Jordanie</country>
<wicri:regionArea>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid</wicri:regionArea>
<wicri:noRegion>Irbid</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Al Jabal, Ghazi Ahmad" sort="Al Jabal, Ghazi Ahmad" uniqKey="Al Jabal G" first="Ghazi-Ahmad" last="Al Jabal">Ghazi-Ahmad Al Jabal</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: gaaljabal12@ph.just.edu.jo.</nlm:affiliation>
<country xml:lang="fr">Jordanie</country>
<wicri:regionArea>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid</wicri:regionArea>
<wicri:noRegion>Irbid</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aissi, Miriem" sort="Aissi, Miriem" uniqKey="Aissi M" first="Miriem" last="Aissi">Miriem Aissi</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aissimiriem@yahoo.fr.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Harhoura, Khaled" sort="Harhoura, Khaled" uniqKey="Harhoura K" first="Khaled" last="Harhoura">Khaled Harhoura</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: harhourakhaled@yahoo.fr.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zenia, Safia" sort="Zenia, Safia" uniqKey="Zenia S" first="Safia" last="Zenia">Safia Zenia</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: safia_zenia@yahoo.fr.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Khammar, Farida" sort="Khammar, Farida" uniqKey="Khammar F" first="Farida" last="Khammar">Farida Khammar</name>
<affiliation wicri:level="1"><nlm:affiliation>Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: faridakhammar@gmail.com.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111</wicri:regionArea>
<wicri:noRegion>16111</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochimie</title>
<idno type="eISSN">1638-6183</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Biomarkers (blood)</term>
<term>Cattle (MeSH)</term>
<term>Chromatography, High Pressure Liquid (methods)</term>
<term>Fasciola hepatica (isolation & purification)</term>
<term>Fascioliasis (diagnosis)</term>
<term>Fascioliasis (veterinary)</term>
<term>Female (MeSH)</term>
<term>Gas Chromatography-Mass Spectrometry (methods)</term>
<term>Male (MeSH)</term>
<term>Pyruvaldehyde (blood)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Bovins (MeSH)</term>
<term>Chromatographie en phase liquide à haute performance (méthodes)</term>
<term>Chromatographie gazeuse-spectrométrie de masse (méthodes)</term>
<term>Fasciola hepatica (isolement et purification)</term>
<term>Fasciolase (diagnostic)</term>
<term>Fasciolase (médecine vétérinaire)</term>
<term>Femelle (MeSH)</term>
<term>Marqueurs biologiques (sang)</term>
<term>Mâle (MeSH)</term>
<term>Méthylglyoxal (sang)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Biomarkers</term>
<term>Pyruvaldehyde</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Fascioliasis</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Fasciolase</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fasciola hepatica</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Fasciola hepatica</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term>
<term>Gas Chromatography-Mass Spectrometry</term>
</keywords>
<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Fasciolase</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term>
<term>Chromatographie gazeuse-spectrométrie de masse</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Marqueurs biologiques</term>
<term>Méthylglyoxal</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Fascioliasis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cattle</term>
<term>Female</term>
<term>Male</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Bovins</term>
<term>Femelle</term>
<term>Mâle</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Two analytical methods; high performance liquid chromatography and gas chromatography were used to determine the content of 2-methylquinoxaline, a methylglyoxal-derived agent in sera from cattle with fascioliasis. Methylglyoxal is a highly mutagenic and cytotoxic reactive dicarbonyl compound formed by non-enzymatic fragmentation of triose phosphate GAP and DHAP during glycolysis which regularly contributes to repositioning the energetic balance between physiological and pathological situations. The aim of this study was to propose the MGO as a new biomarker in the bovine fasciolosis. Strongly infected animals showed a correlation between the relatively high levels of Fasciola hepatica anti-f2 antibody and methylglyoxal compared to unharmed animals. Also, an acute hyperglycemia was recorded and closely related to hepatic parenchyma hyperplasia, inflammation, bile ducts obstruction and scléro-fibrous foci formation.Unlike HPLC, which has shown analytical flaws and irregularities, GC-MS remains an excellent diagnostic tool for detecting and quantifying methylglyoxal in biological fluids. The developed method has been validated under FDA guidelines. A full scan-range was set from m/z 39 to 144/999 and the molecular weight of the 2-methylquinoxaline was identified according to NIST Database and ES. Methylglyoxal was the only analyte successfully quantified in a relatively short run time. It was linear over a concentration range of 0.057-5.7  μg.ml-1with mean recoveries and RSD of 118% and 3.63% respectively. The intra and inter-day assays were satisfying and not exceed 3.00%. Results reflect the degree of precision of our method and indicate that MGO was an important contributor to understand the hepatic failure independently of other serum markers.</div>
</front>
</TEI>
<affiliations><list><country><li>Algérie</li>
<li>Jordanie</li>
</country>
<region><li>Wilaya d'Alger</li>
</region>
<settlement><li>Alger</li>
</settlement>
</list>
<tree><country name="Algérie"><noRegion><name sortKey="Taibi, Nadia" sort="Taibi, Nadia" uniqKey="Taibi N" first="Nadia" last="Taïbi">Nadia Taïbi</name>
</noRegion>
<name sortKey="Abou Mustapha, Mohamed" sort="Abou Mustapha, Mohamed" uniqKey="Abou Mustapha M" first="Mohamed" last="Abou-Mustapha">Mohamed Abou-Mustapha</name>
<name sortKey="Aissi, Miriem" sort="Aissi, Miriem" uniqKey="Aissi M" first="Miriem" last="Aissi">Miriem Aissi</name>
<name sortKey="Ameraoui, Rachid" sort="Ameraoui, Rachid" uniqKey="Ameraoui R" first="Rachid" last="Ameraoui">Rachid Ameraoui</name>
<name sortKey="Boutaiba, Zahra Mouna" sort="Boutaiba, Zahra Mouna" uniqKey="Boutaiba Z" first="Zahra-Mouna" last="Boutaiba">Zahra-Mouna Boutaiba</name>
<name sortKey="Djema, Souhila" sort="Djema, Souhila" uniqKey="Djema S" first="Souhila" last="Djema">Souhila Djema</name>
<name sortKey="Hadjadj, Mohamed" sort="Hadjadj, Mohamed" uniqKey="Hadjadj M" first="Mohamed" last="Hadjadj">Mohamed Hadjadj</name>
<name sortKey="Harhoura, Khaled" sort="Harhoura, Khaled" uniqKey="Harhoura K" first="Khaled" last="Harhoura">Khaled Harhoura</name>
<name sortKey="Kaced, Amel" sort="Kaced, Amel" uniqKey="Kaced A" first="Amel" last="Kaced">Amel Kaced</name>
<name sortKey="Khammar, Farida" sort="Khammar, Farida" uniqKey="Khammar F" first="Farida" last="Khammar">Farida Khammar</name>
<name sortKey="Taibi, Amina" sort="Taibi, Amina" uniqKey="Taibi A" first="Amina" last="Taïbi">Amina Taïbi</name>
<name sortKey="Zenia, Safia" sort="Zenia, Safia" uniqKey="Zenia S" first="Safia" last="Zenia">Safia Zenia</name>
</country>
<country name="Jordanie"><noRegion><name sortKey="Al Balas, Qosay Ali" sort="Al Balas, Qosay Ali" uniqKey="Al Balas Q" first="Qosay-Ali" last="Al-Balas">Qosay-Ali Al-Balas</name>
</noRegion>
<name sortKey="Al Jabal, Ghazi Ahmad" sort="Al Jabal, Ghazi Ahmad" uniqKey="Al Jabal G" first="Ghazi-Ahmad" last="Al Jabal">Ghazi-Ahmad Al Jabal</name>
</country>
</tree>
</affiliations>
</record>

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Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.

Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.

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